Peroxidase-Like Behavior of Ni Thin Films Deposited by Glancing Angle Deposition for Enzyme-Free Uric Acid Sensing.

ABSTRACT: We present a nanozyme-based biosensor fabricated from nanostructured Ni films deposited onto a silicon wafer by glancing angle deposition (GLAD) for enzyme-free colorimetric monitoring of uric acid (UA), a biomarker for gout, high blood pressure, heart disease, and kidney disease. The helically structured Ni GLAD nanozymes exhibit excellent peroxidase-like activity to accelerate the oxidation reaction of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to a blue product, oxidized TMB (oxTMB), mediated by H2O2. In the presence of UA, oxTMB is reduced, decreasing the optical absorbance by an amount determined by the concentration of UA in the solution. The nanozyme not only mimics peroxidase but also possesses the notable qualities of reusability, simple operation, and reliability, making it e

SUBMITTER: Tripathi A 

PROVIDER: S-EPMC7191584 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature