Real-time monitoring of cAMP in brown adipocytes reveals differential compartmentation of β₁ and β₃-adrenoceptor signalling.

ABSTRACT:

Objective

3',5'-Cyclic adenosine monophosphate (cAMP) is a central second messenger governing brown adipocyte differentiation and function. β-adrenergic receptors (β-ARs) stimulate adenylate cyclases which produce cAMP. Moreover, cyclic nucleotide levels are tightly controlled by phosphodiesterases (PDEs), which can generate subcellular microdomains of cAMP. Since the spatio-temporal organisation of the cAMP signalling pathway in adipocytes is still unclear, we sought to monitor real-time cAMP dynamics by live cell imaging in pre-mature and mature brown adipocytes.

Methods

We measured the real-time dynamics of cAMP in murine pre-mature and mature brown adipocytes during stimulation of individual β-AR subtypes, as well as its regulation by PDEs using a Förster Resonance Energ

SUBMITTER: Kannabiran SA 

PROVIDER: S-EPMC7191645 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature