CCR2 is localized in microglia and neurons, as well as infiltrating monocytes, in the lumbar spinal cord of ALS mice.

ABSTRACT: It remains controversial whether circulating monocytes expressing CCR2 infiltrate the central nervous system (CNS) and contribute to pathogenicity of amyotrophic lateral sclerosis (ALS). A previous report used conventional immunohistochemistry to show that CCR2 is exclusively expressed by astrocytes, but not infiltrating monocytes/microglia or neurons, in the spinal cords of ALS model mice. In this study, we assessed the cellular distribution of CCR2 in the CNS of ALS mice using CCR2-reporter mice (Ccr2^rfp/+-Cx3cr1^gfp/+-SOD1^G93A Tg mice), a more sophisticated method for directly detecting the distribution of CCR2 protein. We found that infiltration of CCR2⁺ monocytes in the lumbar spinal cord increased over the course of disease progression. More

SUBMITTER: Komiya H 

PROVIDER: S-EPMC7191738 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature