Increasing the Sensitivity of Electrochemical DNA Detection by a Micropillar-Structured Biosensing Surface.

ABSTRACT: The available active surface area and the density of probes immobilized on this surface are responsible for achieving high specificity and sensitivity in electrochemical biosensors that detect biologically relevant molecules, including DNA. Here, we report the design of gold-coated, silicon micropillar-structured electrodes functionalized with modified poly-l-lysine (PLL) as an adhesion layer to concomitantly assess the increase in sensitivity with the increase of the electrochemical area and control over the probe density. By systematically reducing the center-to-center distance between the pillars (pitch), denser micropillar arrays were formed at the electrode, resulting in a larger sensing area. Azido-modified peptide nucleic acid (PNA) probes were click-reacted onto the electrode inter

SUBMITTER: Movilli J 

PROVIDER: S-EPMC7191753 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature