Dnmt3a and Dnmt3b-Decommissioned Fetal Enhancers are Linked to Kidney Disease.

ABSTRACT:

Background

Cytosine methylation is an epigenetic mark that dictates cell fate and response to stimuli. The timing and establishment of methylation logic during kidney development remains unknown. DNA methyltransferase 3a and 3b are the enzymes capable of establishing de novo methylation.

Methods

We generated mice with genetic deletion of Dnmt3a and Dnmt3b in nephron progenitor cells (Six2^CreDnmt3a/3b) and kidney tubule cells (Ksp^CreDnmt3a/3b). We characterized Ksp^CreDnmt3a/3b mice at baseline and after injury. Unbiased omics profiling, such as whole genome bisulfite sequencing, reduced representation bisulfite sequencing and RNA sequencing were performed on wh

SUBMITTER: Guan Y 

PROVIDER: S-EPMC7191927 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature