A RT-PCR assay for the detection of coronaviruses from four genera.

ABSTRACT:

Background

During the past two decades, three novel coronaviruses (CoVs) have emerged to cause international human epidemics with severe morbidity. CoVs have also emerged to cause severe epidemics in animals. A better understanding of the natural hosts and genetic diversity of CoVs are needed to help mitigate these threats.

Objective

To design and evaluate a molecular diagnostic tool for detection and identification of all currently recognized and potentially future emergent CoVs from the Orthocoronavirinae subfamily.

Study design and results

We designed a semi-nested, reverse transcription RT-PCR assay based upon 38 published genome sequences of human and animal CoVs. We evaluated this assay with 14 human and animal CoVs and 11 other non-CoV respiratory viruses. Thro

SUBMITTER: Xiu L 

PROVIDER: S-EPMC7192118 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature