A GoldenBraid cloning system for synthetic biology in social amoebae.

ABSTRACT: GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.

SUBMITTER: Kundert P 

PROVIDER: S-EPMC7192589 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature