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A GoldenBraid cloning system for synthetic biology in social amoebae.

ABSTRACT: GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.

SUBMITTER: Kundert P 

PROVIDER: S-EPMC7192589 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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A GoldenBraid cloning system for synthetic biology in social amoebae.

Kundert Peter P   Sarrion-Perdigones Alejandro A   Gonzalez Yezabel Y   Katoh-Kurasawa Mariko M   Hirose Shigenori S   Lehmann Peter P   Venken Koen J T KJT   Shaulsky Gad G  

Nucleic acids research 20200501 8

GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent  ...[more]

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