Rapid and assured genetic engineering methods applied to Acinetobacter baylyi ADP1 genome streamlining.

ABSTRACT: One goal of synthetic biology is to improve the efficiency and predictability of living cells by removing extraneous genes from their genomes. We demonstrate improved methods for engineering the genome of the metabolically versatile and naturally transformable bacterium Acinetobacter baylyi ADP1 and apply them to a genome streamlining project. In Golden Transformation, linear DNA fragments constructed by Golden Gate Assembly are directly added to cells to create targeted deletions, edits, or additions to the chromosome. We tested the dispensability of 55 regions of the ADP1 chromosome using Golden Transformation. The 18 successful multiple-gene deletions ranged in size from 21 to 183 kb and collectively accounted for 23.4% of its genome. The success of each multiple-gene deletion attempt c

SUBMITTER: Suarez GA 

PROVIDER: S-EPMC7192602 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature