Enhancing cell seeding and osteogenesis of MSCs on 3D printed scaffolds through injectable BMP2 immobilized ECM-Mimetic gel.

ABSTRACT:

Objective

Design of bioactive scaffolds with osteogenic capacity is a central challenge in cell-based patient-specific bone tissue engineering. Efficient and spatially uniform seeding of (stem) cells onto such constructs is vital to attain functional tissues. Herein we developed heparin functionalized collagen gels supported by 3D printed bioceramic scaffolds, as bone extracellular matrix (ECM)-mimetic matrices. These matrices were designed to enhance cell seeding efficiency of mesenchymal stem cells (MSCs) as well as improve their osteogenic differentiation through immobilized bone morphogenic protein 2 (BMP2) to be used for personalized bone regeneration.

Methods

A 3D gel based on heparin-conjugated collagen matrix capable of immobilizing recombinant human bone morphogenic

SUBMITTER: Fahimipour F 

PROVIDER: S-EPMC7193177 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature