Highly efficient method of Lithospermum erythrorhizon transformation using domestic Rhizobium rhizogenes strain A13.

ABSTRACT: Lithospermum erythrorhizon, a medicinal plant growing in Asian countries, produces shikonin derivatives that are lipophilic secondary metabolites. These red naphthoquinone pigments are traditionally used as a natural drug and a dye in East Asia. In intact L. erythrorhizon plants, shikonin derivatives are produced in the root epidermal cells and secreted into extracellular spaces. The biosynthetic pathway for shikonin derivatives remains incompletely understood and the secretion mechanisms are largely unknown. Understanding the molecular mechanisms underlying shikonin biosynthesis and transport in L. erythrorhizon cells requires functional analysis of candidate genes using transgenic plants. To date, however, standard transformation methods have not yet been established

SUBMITTER: Tatsumi K 

PROVIDER: S-EPMC7193830 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature