Nanodomains can persist at physiologic temperature in plasma membrane vesicles and be modulated by altering cell lipids.

ABSTRACT: The formation and properties of liquid-ordered (Lo) lipid domains (rafts) in the plasma membrane are still poorly understood. This limits our ability to manipulate ordered lipid domain-dependent biological functions. Giant plasma membrane vesicles (GPMVs) undergo large-scale phase separations into coexisting Lo and liquid-disordered lipid domains. However, large-scale phase separation in GPMVs detected by light microscopy is observed only at low temperatures. Comparing Förster resonance energy transfer-detected versus light microscopy-detected domain formation, we found that nanodomains, domains of nanometer size, persist at temperatures up to 20°C higher than large-scale phases, up to physiologic temperature. The persistence of nanodomains at higher temperatures is consistent with previou

SUBMITTER: Li G 

PROVIDER: S-EPMC7193961 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature