HERG K⁺ Channels Promote Survival of Irradiated Leukemia Cells.

ABSTRACT: Many tumor cells express highly elevated activities of voltage-gated K⁺ channels in the plasma membrane which are indispensable for tumor growth. To test for K⁺ channel function during DNA damage response, we subjected human chronic myeloid leukemia (CML) cells to sub-lethal doses of ionizing radiation (0-8 Gy, 6 MV photons) and determined K⁺ channel activity, K⁺ channel-dependent Ca²⁺ signaling, cell cycle progression, DNA repair, and clonogenic survival by whole-cell patch clamp recording, fura-2 Ca²⁺ imaging, Western blotting, flow cytometry, immunofluorescence microscopy, and pre-plating colony formation assay, respectively. As a result, the human erythroid CML cell line K562 and primary human CML cells functionally expr

SUBMITTER: Palme D 

PROVIDER: S-EPMC7194033 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature