CYP72A enzymes catalyse 13-hydrolyzation of gibberellins.

ABSTRACT: Bioactive gibberellins (GAs or diterpenes) are essential hormones in land plants that control many aspects of plant growth and development. In flowering plants, 13-OH GAs (having low bioactivity-for example, GA₁) and 13-H GAs (having high bioactivity-for example, GA₄) frequently coexist in the same plant. However, the identity of the native Arabidopsis thaliana 13-hydroxylase GA and its physiological functions remain unknown. Here, we report that cytochrome P450 genes (CYP72A9 and its homologues) encode active GA 13-hydroxylases in Brassicaceae. Plants overexpressing CYP72A9 exhibited semi-dwarfism, which was caused by significant reduction in GA₄ levels. Biochemical assays revealed that recombinant CYP72A9 protein catalysed the conversion of 13-H GAs to th

SUBMITTER: He J 

PROVIDER: S-EPMC7194175 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature