Sequential phosphoproteomics and N-glycoproteomics of plasma-derived extracellular vesicles.

ABSTRACT: Extracellular vesicles (EVs) are increasingly being recognized as important vehicles for intercellular communication and as promising sources for biomarker discovery. Because the state of protein post-translational modifications (PTMs) such as phosphorylation and glycosylation can be a key determinant of cellular physiology, comprehensive characterization of protein PTMs in EVs can be particularly valuable for early-stage diagnostics and monitoring of disease status. However, the analysis of PTMs in EVs has been complicated by limited amounts of purified EVs, low-abundance PTM proteins, and interference from proteins and metabolites in biofluids. Recently, we developed an approach to isolate phosphoproteins and glycoproteins in EVs from small volumes of human plasma that enabled us to iden

SUBMITTER: Andaluz Aguilar H 

PROVIDER: S-EPMC7194202 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature