Quantitative and correlative extreme ultraviolet coherent imaging of mouse hippocampal neurons at high resolution.

ABSTRACT: Microscopy with extreme ultraviolet (EUV) light can provide many advantages over optical, hard x-ray or electron-based techniques. However, traditional EUV sources and optics have large disadvantages of scale and cost. Here, we demonstrate the use of a laboratory-scale, coherent EUV source to image biological samples-mouse hippocampal neurons-providing quantitative phase and amplitude transmission information with a lateral resolution of 80 nm and an axial sensitivity of ~1 nm. A comparison with fluorescence imaging of the same samples demonstrated EUV imaging was able to identify, without the need for staining or superresolution techniques, <100-nm-wide and <10-nm-thick structures not observable from the fluorescence images. Unlike hard x-ray microscopy, no damage is observed of the delic

SUBMITTER: Baksh PD 

PROVIDER: S-EPMC7195139 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature