Engineering of human cardiac muscle electromechanically matured to an adult-like phenotype.

ABSTRACT: The application of tissue-engineering approaches to human induced pluripotent stem (hiPS) cells enables the development of physiologically relevant human tissue models for in vitro studies of development, regeneration, and disease. However, the immature phenotype of hiPS-derived cardiomyocytes (hiPS-CMs) limits their utility. We have developed a protocol to generate engineered cardiac tissues from hiPS cells and electromechanically mature them toward an adult-like phenotype. This protocol also provides optimized methods for analyzing these tissues' functionality, ultrastructure, and cellular properties. The approach relies on biological adaptation of cultured tissues subjected to biomimetic cues, applied at an increasing intensity, to drive accelerated maturation. hiPS cells are differenti

SUBMITTER: Ronaldson-Bouchard K 

PROVIDER: S-EPMC7195192 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature