Quantitating Endosomal Escape of a Library of Polymers for mRNA Delivery.

ABSTRACT: Endosomal escape is a key step for intracellular drug delivery of nucleic acids, but reliable and sensitive methods for its quantitation remain an unmet need. In order to rationally optimize the mRNA transfection efficiency of a library of polymeric materials, we designed a deactivated Renilla luciferase-derived molecular probe whose activity can be restored only in the cytosol. This probe can be coencapsulated with mRNA in the same delivery vehicle, thereby accurately measuring its endosomal escape efficiency. We examined a library of poly(amine-co-ester) (PACE) polymers with different end groups using this probe and observed a strong correlation between endosomal escape and transfection efficiency (R² = 0.9334). In addition, we found that mRNA encapsulation effic

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC7195212 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature