Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization.

ABSTRACT: The human genome is extensively folded into 3-dimensional organization. However, the detailed 3D chromatin folding structures have not been fully visualized due to the lack of robust and ultra-resolution imaging capability. Here, we report the development of an electron microscopy method that combines serial block-face scanning electron microscopy with in situ hybridization (3D-EMISH) to visualize 3D chromatin folding at targeted genomic regions with ultra-resolution (5 × 5 × 30 nm in xyz dimensions) that is superior to the current super-resolution by fluorescence light microscopy. We apply 3D-EMISH to human lymphoblastoid cells at a 1.7 Mb segment of the genome and visualize a large number of distinctive 3D chromatin folding structures in ultra-resolution. We further quantitatively charac

SUBMITTER: Trzaskoma P 

PROVIDER: S-EPMC7195386 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature