Dynamics of within-host Mycobacterium tuberculosis diversity and heteroresistance during treatment.

ABSTRACT:

Background

Studying within-host genetic diversity of Mycobacterium tuberculosis (Mtb) in patients during treatment may identify adaptations to antibiotic and immune pressure. Understanding the significance of genetic heteroresistance, and more specifically heterozygous resistance-associated variants (RAVs), is clinically important given increasing use of rapid molecular tests and whole genome sequencing (WGS).

Methods

We analyse data from six studies in KwaZulu-Natal, South Africa. Most patients (>75%) had baseline rifampicin resistance. Sputum was collected for culture at baseline and at between two and nine intervals until month six. Positive cultures underwent WGS. Mixed infections and reinfections were excluded from analysis.

Findings

Baseline Mtb overall genetic

SUBMITTER: Nimmo C 

PROVIDER: S-EPMC7195533 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature