Host transcriptomic signature as alternative test-of-cure in visceral leishmaniasis patients co-infected with HIV.

ABSTRACT:

Background

Visceral leishmaniasis (VL) treatment in HIV patients very often fails and is followed by high relapse and case-fatality rates. Hence, treatment efficacy assessment is imperative but based on invasive organ aspiration for parasite detection. In the search of a less-invasive alternative and because the host immune response is pivotal for treatment outcome in immunocompromised VL patients, we studied changes in the whole blood transcriptional profile of VL-HIV patients during treatment.

Methods

Embedded in a clinical trial in Northwest Ethiopia, RNA-Seq was performed on whole blood samples of 28 VL-HIV patients before and after completion of a 29-day treatment regimen of AmBisome or AmBisome/miltefosine. Pathway analyses were combined with a machine learning approac

SUBMITTER: Adriaensen W 

PROVIDER: S-EPMC7195535 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature