Evaluation of the expression levels of BRAF^V600E mRNA in primary tumors of thyroid cancer using an ultrasensitive mutation assay.

ABSTRACT:

Background

The BRAF^V600E gene encodes for the mutant BRAF^V600E protein, which triggers downstream oncogenic signaling in thyroid cancer. Since most currently available methods have focused on detecting BRAF^V600E mutations in tumor DNA, there is limited information about the level of BRAF^V600E mRNA in primary tumors of thyroid cancer, and the diagnostic relevance of these RNA mutations is not known.

Methods

Sixty-two patients with thyroid cancer and non-malignant thyroid disease were included in the study. Armed with an ultrasensitive technique for mRNA-based mutation analysis based on a two step RT-qPCR method, we analysed the expression levels of the mutated BRAF^V600E mRNA in formalin-fixed paraffin-embedded samples of

SUBMITTER: Tran TV 

PROVIDER: S-EPMC7195771 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature