Dihydrotestosterone suppression of proinflammatory gene expression in human meibomian gland epithelial cells.

ABSTRACT:

Purpose

We discovered that dihydrotestosterone (DHT) decreases the ability of lipopolysaccharide, a bacterial toxin, to stimulate the secretion of leukotriene B4, a potent proinflammatory mediator, by immortalized human meibomian gland epithelial cells (IHMGECs). We hypothesize that this hormone action reflects an androgen suppression of proinflammatory gene activity in these cells. Our goal was to test this hypothesis. For comparison, we also examined whether DHT treatment elicits the same effect in immortalized human corneal (IHC) and conjunctival (IHConj) ECs.

Methods

Differentiated cells were cultured in media containing vehicle or 10 nM DHT. Cells (n = 3 wells/treatment group) were then processed for RNA isolation and the analysis of gene expression by using Illumina Be

SUBMITTER: Sahin A 

PROVIDER: S-EPMC7195874 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature