Dataset Information

Reliable blood cancer cells' telomere length evaluation by qPCR.

ABSTRACT:

Background

Telomere shortening is linked to a range of different human diseases, hence reliable measurement methods are needed to uncover such associations. Among the plethora of telomere length measurement methods, qPCR is reported as easy to conduct and a cost-effective approach to study samples with low DNA amounts.

Methods

Cancer cells' telomere length was evaluated by relative and absolute qPCR methods.

Results

Robust and reproducible telomere length measurements were optimized taking into account a careful reference gene selection and by knowing the cancer cells ploidy. qPCR data were compared to "gold standard" measurement from terminal restriction fragment (TRF).