Dataset Information
Reliable blood cancer cells' telomere length evaluation by qPCR.
Ontology highlight
ABSTRACT: Background
Telomere shortening is linked to a range of different human diseases, hence reliable measurement methods are needed to uncover such associations. Among the plethora of telomere length measurement methods, qPCR is reported as easy to conduct and a cost-effective approach to study samples with low DNA amounts.Methods
Cancer cells' telomere length was evaluated by relative and absolute qPCR methods.Results
Robust and reproducible telomere length measurements were optimized taking into account a careful reference gene selection and by knowing the cancer cells ploidy. qPCR data were compared to "gold standard" measurement from terminal restriction fragment (TRF).Conclusions
Our study provides guidance and recommendations for accurate telomere length measurement by qPCR in cancer cells, taking advantage of our expertise in telomere homeostasis investigation in primary cutaneous T-cell lymphomas. Furthermore, our data emphasize the requirement of samples with both, high DNA quality and high tumor cells representation.
SUBMITTER: Ropio J
PROVIDER: S-EPMC7196062 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Publications
Reliable blood cancer cells' telomere length evaluation by qPCR.
Cancer medicine 20200306 9
<h4>Background</h4>Telomere shortening is linked to a range of different human diseases, hence reliable measurement methods are needed to uncover such associations. Among the plethora of telomere length measurement methods, qPCR is reported as easy to conduct and a cost-effective approach to study samples with low DNA amounts.<h4>Methods</h4>Cancer cells' telomere length was evaluated by relative and absolute qPCR methods.<h4>Results</h4>Robust and reproducible telomere length measurements were ...[more]