Role of dopamine and selective dopamine receptor agonists on mouse ductus arteriosus tone and responsiveness.

ABSTRACT:

Background

Indomethacin treatment for patent ductus arteriosus (PDA) is associated with acute kidney injury (AKI). Fenoldopam, a dopamine (DA) DA₁-like receptor agonist dilates the renal vasculature and may preserve renal function during indomethacin treatment. However, limited information exists on DA receptor-mediated signaling in the ductus and fenoldopam may prevent ductus closure given its vasodilatory nature.

Methods

DA receptor expression in CD-1 mouse vessels was analyzed by qPCR and immunohistochemistry. Concentration-response curves were established using pressure myography. Pretreatment with SCH23390 (DA₁-like receptor antagonist), phentolamine (α -adrenergic receptor antagonist) or indomethacin addressed mechanisms for DA-induced changes. Fe

SUBMITTER: Crockett SL 

PROVIDER: S-EPMC7196482 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature