Proximity Dependent Biotinylation: Key Enzymes and Adaptation to Proteomics Approaches.

ABSTRACT: The study of protein subcellular distribution, their assembly into complexes and the set of proteins with which they interact with is essential to our understanding of fundamental biological processes. Complementary to traditional assays, proximity-dependent biotinylation (PDB) approaches coupled with mass spectrometry (such as BioID or APEX) have emerged as powerful techniques to study proximal protein interactions and the subcellular proteome in the context of living cells and organisms. Since their introduction in 2012, PDB approaches have been used in an increasing number of studies and the enzymes themselves have been subjected to intensive optimization. How these enzymes have been optimized and considerations for their use in proteomics experiments are important questions. Here, we r

SUBMITTER: Samavarchi-Tehrani P 

PROVIDER: S-EPMC7196579 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature