Zinc promotes liquid-liquid phase separation of tau protein.

ABSTRACT: Tau is a microtubule-associated protein that plays a major role in Alzheimer's disease (AD) and other tauopathies. Recent reports indicate that, in the presence of crowding agents, tau can undergo liquid-liquid phase separation (LLPS), forming highly dynamic liquid droplets. Here, using recombinantly expressed proteins, turbidimetry, fluorescence microscopy imaging, and fluorescence recovery after photobleaching (FRAP) assays, we show that the divalent transition metal zinc strongly promotes this process, shifting the equilibrium phase boundary to lower protein or crowding agent concentrations. We observed no tau LLPS-promoting effect for any other divalent transition metal ions tested, including Mn²⁺, Fe²⁺, Co²⁺, Ni²⁺, and Cu²⁺ We als

SUBMITTER: Singh V 

PROVIDER: S-EPMC7196643 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature