Robust landscapes of ribosome dwell times and aminoacyl-tRNAs in response to nutrient stress in liver.

ABSTRACT: Translation depends on messenger RNA (mRNA)-specific initiation, elongation, and termination rates. While translation elongation is well studied in bacteria and yeast, less is known in higher eukaryotes. Here we combined ribosome and transfer RNA (tRNA) profiling to investigate the relations between translation elongation rates, (aminoacyl-) tRNA levels, and codon usage in mammals. We modeled codon-specific ribosome dwell times from ribosome profiling, considering codon pair interactions between ribosome sites. In mouse liver, the model revealed site- and codon-specific dwell times that differed from those in yeast, as well as pairs of adjacent codons in the P and A site that markedly slow down or speed up elongation. While translation efficiencies vary across diurnal time and feeding regi

SUBMITTER: Gobet C 

PROVIDER: S-EPMC7196831 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature