Separase activity distribution can be a marker of major molecular response and proliferation of CD34⁺ cells in TKI-treated chronic myeloid leukemia patients.

ABSTRACT: Separase, a cysteine endopeptidase, is a key player in mitotic sister chromatid separation, replication fork dynamics, and DNA repair. Aberrant expression and/or altered separase proteolytic activity are associated with aneuploidy, tumorigenesis, and disease progression. Since genomic instability and clonal evolution are hallmarks of progressing chronic myeloid leukemia (CML), we have comparatively examined separase proteolytic activity in TKI-treated chronic phase CML. Separase proteolytic activity was analyzed on single cell level in 88 clinical samples and in 14 healthy controls by a flow cytometric assay. In parallel, BCR-ABL1 gene expression and replication fork velocity were measured by qRT-PCR and DNA fiber assays, respectively. The separase activity distribution (SAD) value indicat

SUBMITTER: Spiess B 

PROVIDER: S-EPMC7196950 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature