High-throughput method for detection and quantification of lesions on leaf scale based on trypan blue staining and digital image analysis.

ABSTRACT:

Background

Field-grown leafy vegetables can be damaged by biotic and abiotic factors, or mechanically damaged by farming practices. Available methods to evaluate leaf tissue damage mainly rely on colour differentiation between healthy and damaged tissues. Alternatively, sophisticated equipment such as microscopy and hyperspectral cameras can be employed. Depending on the causal factor, colour change in the wounded area is not always induced and, by the time symptoms become visible, a plant can already be severely affected. To accurately detect and quantify damage on leaf scale, including microlesions, reliable differentiation between healthy and damaged tissue is essential. We stained whole leaves with trypan blue dye, which traverses compromised cell membranes but is not absorbed

SUBMITTER: Mulaosmanovic E 

PROVIDER: S-EPMC7197134 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature