Toll-like receptors 2 and 7 mediate coagulation activation and coagulopathy in murine sepsis.

ABSTRACT:

Background

Sepsis is a life-threatening condition often manifested as marked inflammation and severe coagulopathy. Toll-like receptors (TLRs) play a pivotal role in inflammation, organ dysfunction and mortality in animal sepsis.

Objectives

To investigate the role of TLR signaling in mediating sepsis-induced coagulopathy (SIC) in a mouse model.

Methods

Polymicrobial sepsis was created by cecal ligation and puncture (CLP) or fecal slurry peritoneal injection. To quantify global clotting function, two viscoelastic assays were performed with rotational thromboelastometry, and the results were presented as maximum clot firmness (MCF): (a) EXTEM to test tissue factor (TF)-initiated clot formation; and (b) FIBTEM to test EXTEM in the presence of a platelet inhibitor, cytocha

SUBMITTER: Williams B 

PROVIDER: S-EPMC7197442 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature