RETrace: simultaneous retrospective lineage tracing and methylation profiling of single cells.

ABSTRACT: Retrospective lineage tracing harnesses naturally occurring mutations in cells to elucidate single cell development. Common single-cell phylogenetic fate mapping methods have utilized highly mutable microsatellite loci found within the human genome. Such methods were limited by the introduction of in vitro noise through polymerase slippage inherent in DNA amplification, which we characterized to be approximately 10-100× higher than the in vivo replication mutation rate. Here, we present RETrace, a method for simultaneously capturing both microsatellites and methylation-informative cytosines to characterize both lineage and cell type, respectively, from the same single cell. An important unique feature of RETrace was the introduction of linear amplification of microsatellites in order to re

SUBMITTER: Wei CJ 

PROVIDER: S-EPMC7197472 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature