Inhibition of transcription leads to rewiring of locus-specific chromatin proteomes.

ABSTRACT: Transcription of a chromatin template involves the concerted interaction of many different proteins and protein complexes. Analyses of specific factors showed that these interactions change during stress and upon developmental switches. However, how the binding of multiple factors at any given locus is coordinated has been technically challenging to investigate. Here we used Epi-Decoder in yeast to systematically decode, at one transcribed locus, the chromatin binding changes of hundreds of proteins in parallel upon perturbation of transcription. By taking advantage of improved Epi-Decoder libraries, we observed broad rewiring of local chromatin proteomes following chemical inhibition of RNA polymerase. Rapid reduction of RNA polymerase II binding was accompanied by reduced binding of many

SUBMITTER: Poramba-Liyanage DW 

PROVIDER: S-EPMC7197482 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature