Runx1 promotes scar deposition and inhibits myocardial proliferation and survival during zebrafish heart regeneration.

ABSTRACT: Runx1 is a transcription factor that plays a key role in determining the proliferative and differential state of multiple cell types, during both development and adulthood. Here, we report how Runx1 is specifically upregulated at the injury site during zebrafish heart regeneration, and that absence of runx1 results in increased myocardial survival and proliferation, and overall heart regeneration, accompanied by decreased fibrosis. Using single cell sequencing, we found that the wild-type injury site consists of Runx1-positive endocardial cells and thrombocytes that induce expression of smooth muscle and collagen genes. Both these populations cannot be identified in runx1 mutant wounds that contain less collagen and fibrin. The reduction in fibrin in the mutant is further exp

SUBMITTER: Koth J 

PROVIDER: S-EPMC7197712 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature