Ontology highlight
ABSTRACT:
SUBMITTER: Cao X
PROVIDER: S-EPMC7197872 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Journal of cell science 20200430 8
Folding of proteins entering the mammalian secretory pathway requires the insertion of the correct disulfides. Disulfide formation involves both an oxidative pathway for their insertion and a reductive pathway to remove incorrectly formed disulfides. Reduction of these disulfides is crucial for correct folding and degradation of misfolded proteins. Previously, we showed that the reductive pathway is driven by NADPH generated in the cytosol. Here, by reconstituting the pathway using purified prot ...[more]