Plasma Formate Is Greater in Fetal and Neonatal Rats Compared with Their Mothers.

ABSTRACT:

Background

Formate can be incorporated into 10-formyl-tetrahydrofolate (10-formyl-THF), which is a substrate for purine synthesis, and after further reduction of the one-carbon group, may be used as a substrate for thymidylate synthesis and for homocysteine remethylation.

Objective

We examined plasma formate concentrations and the expression of genes involved in the production and utilization of formate in fetal and neonatal rats and in pregnant and virgin female rats.

Methods

In 1 experiment, plasma formate was measured by GC-MS in rats aged 1-56 d. In a second experiment, virgin female (adult) rats, 19-d pregnant rats (P) and their male and female fetuses (F), and 3-d-old (N) and 7-d-old (J) offspring had plasma and amniotic fluid analyzed for formate by GC-MS, mRNA

SUBMITTER: Brosnan ME 

PROVIDER: S-EPMC7198295 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature