PC4 serves as a negative regulator of skin wound healing in mice.

ABSTRACT:

Background

Human positive cofactor 4 (PC4) was initially characterized as a multifunctional transcriptional cofactor, but its role in skin wound healing is still unclear. The purpose of this study was to explore the role of PC4 in skin wound healing through PC4 knock-in mouse model.

Methods

A PC4 knock-in mouse model (PC4^+/+) with a dorsal full-thickness wound was used to investigate the biological functions of PC4 in skin wound healing. Quantitative PCR, Western blot analysis and immunohistochemistry were performed to evaluate the expression of PC4; Sirius red staining and immunofluorescence were performed to explore the change of collagen deposition and angiogenesis. Proliferation and apoptosis were detected using Ki67 staining and TUNEL assay. Primary dermal fi

SUBMITTER: Liao F 

PROVIDER: S-EPMC7198317 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature