A rapid and simple fluorescence enzyme-linked immunosorbent assay for tetrabromobisphenol A in soil samples based on a bifunctional fusion protein.

ABSTRACT: Tetrabromobisphenol A (TBBPA) is the largest brominated flame retardant which can be released to environment and cause long-term hazard. In this work, we developed a rapid and highly sensitive fluorescence enzyme-linked immunosorbent assay (FELISA) for monitoring of TBBPA in soil samples. TBBPA specific nanobody derived from camelid was fused with alkaline phosphatase to obtain the bi-functional fusion protein, which enable the specific binding of TBBPA and the generation of detection signal simultaneously. The assay showed an IC₅₀ of 0.23 ng g^-1, limit detection of 0.05 ng g^-1 and linear range from 0.1 to 0.55 ng g^-1 for TBBPA in soil samples. Due to the high resistance to organic solvents of the fusion protein, a simple pre-treatment by using 4

SUBMITTER: Fu HJ 

PROVIDER: S-EPMC7198468 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature