Nitrogen Substrate Utilization in Three Rhizosphere Bacterial Strains Investigated Using Proteomics.

ABSTRACT: Nitrogen metabolism in the rhizosphere microbiome plays an important role in mediating plant nutrition, particularly under low inputs of mineral fertilizers. However, there is relatively little mechanistic information about which genes and metabolic pathways are induced by rhizosphere bacterial strains to utilize diverse nitrogen substrates. Here we investigate nitrogen substrate utilization in three taxonomically diverse bacterial strains previously isolated from Arabidopsis roots. The three strains represent taxa that are consistently detected as core members of the plant microbiome: Pseudomonas, Streptomyces, and Rhizobium. We use phenotype microarrays to determine the nitrogen substrate preferences of these strains, and compare the experimental results vs. computational simulations of

SUBMITTER: Jacoby RP 

PROVIDER: S-EPMC7198800 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature