Kinetic Investigation of Translesion Synthesis across a 3-Nitrobenzanthrone-Derived DNA Lesion Catalyzed by Human DNA Polymerase Kappa.

ABSTRACT: 3-Nitrobenzanthrone (3-NBA) is a byproduct of diesel exhaust and is highly present in industrial and populated areas. Inhalation of 3-NBA results in formation of N-(2'-deoxyguanosin-8-yl)-3-aminobenzanthrone (dG^C8-N-ABA), a bulky DNA lesion that is of concern due to its mutagenic and carcinogenic potential. If dG^C8-N-ABA is not bypassed during genomic replication, the lesion can stall cellular DNA replication machinery, leading to senescence or apoptosis. We have previously used running start assays to demonstrate that human DNA polymerases eta (hPolη) and kappa (hPolκ) are able to catalyze translesion DNA synthesis (TLS) across a site-specifically placed dG^C8-N-ABA in a DNA template. Consistently, gene knockdown of hPolη and hPo

SUBMITTER: Phi KK 

PROVIDER: S-EPMC7198833 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature