Identification of preexisting adaptive immunity to Cas9 proteins in humans.

ABSTRACT: The CRISPR-Cas9 system is a powerful tool for genome editing, which allows the precise modification of specific DNA sequences. Many efforts are underway to use the CRISPR-Cas9 system to therapeutically correct human genetic diseases^1-6. The most widely used orthologs of Cas9 are derived from Staphylococcus aureus and Streptococcus pyogenes^5,7. Given that these two bacterial species infect the human population at high frequencies^8,9, we hypothesized that humans may harbor preexisting adaptive immune responses to the Cas9 orthologs derived from these bacterial species, SaCas9 (S. aureus) and SpCas9 (S. pyogenes). By probing human serum for the presence of anti-Cas9 antibodies using an enzyme-linked immunosorbent assay, we detected antibodies against both SaCa

SUBMITTER: Charlesworth CT 

PROVIDER: S-EPMC7199589 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature