Fluorescent reporter plasmids for single-cell and bulk-level composition assays in E. faecalis.

ABSTRACT: Fluorescent reporters are an important tool for monitoring dynamics of bacterial populations at the single cell and community level. While there are a large range of reporter constructs available-particularly for common model organisms like E. coli-fewer options exist for other species, including E. faecalis, a gram-positive opportunistic pathogen. To expand the potential toolkit available for E. faecalis, we exchanged the original fluorescent reporter in a previously developed plasmid (pBSU101) with one of eight fluorescent reporters and confirmed that all constructs exhibited detectable fluorescence in single E. faecalis cells and mixed biofilm communities. To identify promising constructs for bulk-level experiments, we then measured the fluorescence spectra from E. faecalis populations

SUBMITTER: Hallinen KM 

PROVIDER: S-EPMC7199960 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature