Cryo-EM structure of the potassium-chloride cotransporter KCC4 in lipid nanodiscs.

ABSTRACT: Cation-chloride-cotransporters (CCCs) catalyze transport of Cl^- with K⁺ and/or Na⁺across cellular membranes. CCCs play roles in cellular volume regulation, neural development and function, audition, regulation of blood pressure, and renal function. CCCs are targets of clinically important drugs including loop diuretics and their disruption has been implicated in pathophysiology including epilepsy, hearing loss, and the genetic disorders Andermann, Gitelman, and Bartter syndromes. Here we present the structure of a CCC, the Mus musculus K⁺-Cl^- cotransporter (KCC) KCC4, in lipid nanodiscs determined by cryo-EM. The structure, captured in an inside-open conformation, reveals the architecture of KCCs including an extracellular domai

SUBMITTER: Reid MS 

PROVIDER: S-EPMC7200160 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature