High-dimensional analyses reveal a distinct role of T-cell subsets in the immune microenvironment of gastric cancer.

ABSTRACT:

Objectives

To facilitate disease prognosis and improve precise immunotherapy of gastric cancer (GC) patients, a comprehensive study integrating immune cellular and molecular analyses on tumor tissues and peripheral blood was performed.

Methods

The association of GC patients' outcomes and the immune context of their tumors was explored using multiplex immunohistochemistry (mIHC) and transcriptome profiling. Potential immune dysfunction mechanism/s in the tumors on the systemic level was further examined using mass cytometry (CyTOF) in complementary peripheral blood from selected patients. GC cohorts with mIHC and gene expression profiling data were also used as validation cohorts.

Results

Increased CD4⁺FOXP3⁺ T-cell density in the GC tumor correla

SUBMITTER: Wang M 

PROVIDER: S-EPMC7200219 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature