Pipeline for the removal of hardware related artifacts and background noise for Raman spectroscopy.

ABSTRACT: Raman spectroscopy is a real-time, non-contact, and non-destructive technique able to obtain information about the composition of materials, chemicals, and mixtures. It uses the energy transfer properties of molecules to detect the composition of matter. Raman spectroscopy is mainly used in the chemical field because background fluorescence and instrumental noise affect biological (in vitro and in vivo) measurements. In this method, we describe how hardware related artifacts and fluorescence background can be corrected without affecting signal of the measurement. First, we applied manual correction for cosmic ray spikes, followed by automated correction to reduce fluorescence and hardware related artifacts based on a partial 5th degree polynomial fitting and Tophat correction. Along with t

SUBMITTER: Bertens CJF 

PROVIDER: S-EPMC7200319 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature