Genetic tool development in marine protists: emerging model organisms for experimental cell biology.
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ABSTRACT:
SUBMITTER: Faktorova D
PROVIDER: S-EPMC7200600 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Publications
Genetic tool development in marine protists: emerging model organisms for experimental cell biology.
Faktorová Drahomíra D Nisbet R Ellen R RER Fernández Robledo José A JA Casacuberta Elena E Sudek Lisa L Allen Andrew E AE Ares Manuel M Aresté Cristina C Balestreri Cecilia C Barbrook Adrian C AC Beardslee Patrick P Bender Sara S Booth David S DS Bouget François-Yves FY Bowler Chris C Breglia Susana A SA Brownlee Colin C Burger Gertraud G Cerutti Heriberto H Cesaroni Rachele R Chiurillo Miguel A MA Clemente Thomas T Coles Duncan B DB Collier Jackie L JL Cooney Elizabeth C EC Coyne Kathryn K Docampo Roberto R Dupont Christopher L CL Edgcomb Virginia V Einarsson Elin E Elustondo Pía A PA Federici Fernan F Freire-Beneitez Veronica V Freyria Nastasia J NJ Fukuda Kodai K García Paulo A PA Girguis Peter R PR Gomaa Fatma F Gornik Sebastian G SG Guo Jian J Hampl Vladimír V Hanawa Yutaka Y Haro-Contreras Esteban R ER Hehenberger Elisabeth E Highfield Andrea A Hirakawa Yoshihisa Y Hopes Amanda A Howe Christopher J CJ Hu Ian I Ibañez Jorge J Irwin Nicholas A T NAT Ishii Yuu Y Janowicz Natalia Ewa NE Jones Adam C AC Kachale Ambar A Fujimura-Kamada Konomi K Kaur Binnypreet B Kaye Jonathan Z JZ Kazana Eleanna E Keeling Patrick J PJ King Nicole N Klobutcher Lawrence A LA Lander Noelia N Lassadi Imen I Li Zhuhong Z Lin Senjie S Lozano Jean-Claude JC Luan Fulei F Maruyama Shinichiro S Matute Tamara T Miceli Cristina C Minagawa Jun J Moosburner Mark M Najle Sebastián R SR Nanjappa Deepak D Nimmo Isabel C IC Noble Luke L Novák Vanclová Anna M G AMG Nowacki Mariusz M Nuñez Isaac I Pain Arnab A Piersanti Angela A Pucciarelli Sandra S Pyrih Jan J Rest Joshua S JS Rius Mariana M Robertson Deborah D Ruaud Albane A Ruiz-Trillo Iñaki I Sigg Monika A MA Silver Pamela A PA Slamovits Claudio H CH Jason Smith G G Sprecher Brittany N BN Stern Rowena R Swart Estienne C EC Tsaousis Anastasios D AD Tsypin Lev L Turkewitz Aaron A Turnšek Jernej J Valach Matus M Vergé Valérie V von Dassow Peter P von der Haar Tobias T Waller Ross F RF Wang Lu L Wen Xiaoxue X Wheeler Glen G Woods April A Zhang Huan H Mock Thomas T Worden Alexandra Z AZ Lukeš Julius J
Nature methods 20200406 5
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never be ...[more]