Differential effects of 'resurrecting' Csp pseudoproteases during Clostridioides difficile spore germination.

ABSTRACT: Clostridioides difficile is a spore-forming bacterial pathogen that is the leading cause of hospital-acquired gastroenteritis. C. difficile infections begin when its spore form germinates in the gut upon sensing bile acids. These germinants induce a proteolytic signaling cascade controlled by three members of the subtilisin-like serine protease family, CspA, CspB, and CspC. Notably, even though CspC and CspA are both pseudoproteases, they are nevertheless required to sense germinants and activate the protease, CspB. Thus, CspC and CspA are part of a growing list of pseudoenzymes that play important roles in regulating cellular processes. However, despite their importance, the structural properties of pseudoenzymes that allow them to function as regulators remain poorly understood. Our rece

SUBMITTER: Donnelly ML 

PROVIDER: S-EPMC7200643 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature