Segregation of an MSH1 RNAi transgene produces heritable non-genetic memory in association with methylome reprogramming.

ABSTRACT: MSH1 is a plant-specific protein. RNAi suppression of MSH1 results in phenotype variability for developmental and stress response pathways. Segregation of the RNAi transgene produces non-genetic msh1 'memory' with multi-generational inheritance. First-generation memory versus non-memory comparison, and six-generation inheritance studies, identifies gene-associated, heritable methylation repatterning. Genome-wide methylome analysis integrated with RNAseq and network-based enrichment studies identifies altered circadian clock networks, and phytohormone and stress response pathways that intersect with circadian control. A total of 373 differentially methylated loci comprising these networks are sufficient to discriminate memory from nonmemory full sibs. Methylation inhibitor 5-azacytidine dim

SUBMITTER: Yang X 

PROVIDER: S-EPMC7200659 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature