Development of a new macrophage-specific TRAP mouse (Mac^TRAP) and definition of the renal macrophage translational signature.

ABSTRACT: Tissue macrophages play an important role in organ homeostasis, immunity and the pathogenesis of various inflammation-driven diseases. One major challenge has been to selectively study resident macrophages in highly heterogeneous organs such as kidney. To address this problem, we adopted a Translational Ribosome Affinity Purification (TRAP)- approach and designed a transgene that expresses an eGFP-tagged ribosomal protein (L10a) under the control of the macrophage-specific c-fms promoter to generate c-fms-eGFP-L10a transgenic mice (Mac^TRAP). Rigorous characterization found no gross abnormalities in Mac^TRAP mice and confirmed transgene expression across various organs. Immunohistological analyses of Mac^TRAP kidneys identified eGFP-L10a expressing cells in th

SUBMITTER: Hofmeister A 

PROVIDER: S-EPMC7200716 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature