Characterisation of unessential genes required for survival under conditions of DNA stress.

ABSTRACT: BACKGROUND:Genomic instability is a hallmark of cancer. Cancer progression depends on the development and amplification of mutations that alter the cellular response to threats to the genome. This can lead to DNA replication stress and the potential loss of genetic integrity of the newly formed cells. This study utilised fission yeast to map the interactions occurring in some of the most crucial pathways in both DNA replication and checkpoint monitoring involving Rad4, the Schizosaccharomyces pombe (S. pombe) TopBP1 homologue. We have modelled conditions of replication stress in the genetically tractable fission yeast, S. pombe using the hypomorphic rad4-116 allele. Synthetic genetic analysis was used to identify processes required for cell survival under conditions of DNA replication stre

SUBMITTER: Ahmed Ezzat H 

PROVIDER: S-EPMC7201005 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature