Anti-inflammatory effects of glycine thymosin β4 eye drops in experimental dry eye.

ABSTRACT: The aim of the present study was to investigate the anti-inflammatory effects of glycine thymosin β4 (Gly-Tβ4) eye drops, and to compare the efficacy of topical Gly-Tβ4 with Cyclosporine A (CsA) in a mouse model of experimental dry eye (EDE). Eye drops consisting of balanced salt solution (BSS), 0.1% Gly-Tβ4 or 0.05% CsA were used for treatment of EDE. Tear volume, tear film break-up time and corneal staining scores were measured after 7 and 14 days. Periodic acid-Schiff staining for conjunctival gobleT cells, TUNEL assay for corneal apoptotic positive cells, multiplex immunobead assay for interleukin (IL)-1β, IL-6, tumor necrosis factor-α and interferon-γ levels, and flow cytometry for CD4⁺/CCR5⁺ T cells were performed after 14 days. All clinical parameters showed im

SUBMITTER: Jin R 

PROVIDER: S-EPMC7201140 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature